Kremer
In this research project, we conducted a comprehensive investigation into the structural model of major and minor ampullate spider silk, aiming to unravel their mechanical properties. By employing a high-pressure setup, we performed pressure-dependent measurements and spectroscopic analysis on the silk samples. Notably, we observed significant spectral shifts in the vibration frequency of polyalanine blocks, which provided valuable insights into the stability of β-sheet nanocrystals and their interplay with the amorphous phase within the silk structure. These findings supported a refined structural model of spider silk, highlighting a red shift in vibration frequency under stress and a blue shift under hydrostatic pressure.
Furthermore, we developed a novel approach that combined spectral dichroism with microscopy images to assess the pressure dependence of the molecular order parameter in spider silk. This innovative technique allowed us to probe the changes in molecular orientation within the silk structure under different pressure conditions. Interestingly, our investigation of minor ampullate silk revealed similar pressure-dependent behavior, albeit without a distinct kink. Complementary wide-angle x-ray scattering experiments provided additional evidence in support of our refined structural model.
The outcomes of this research project significantly contribute to our understanding of spider silk’s structural properties and its response to various mechanical and environmental factors. By elucidating the mechanical behavior of major and minor ampullate spider silk, our findings open up new avenues for the design and development of biomimetic materials with exceptional mechanical characteristics and applications in fields such as materials science, bioengineering, and nanotechnology.
Highlighted Publications:
- Ene R., P. Papadopoulos, F. Kremer, Supercontraction in Nephila spider dragline silk – Relaxation into equilibrium state, Polymer 52, 6056–6060 (2011)
- Anton M., W. Kossack, C. Gutsche, R. Figuli (Ene), P. Papadopoulos, J. Ebad-Allah, C. Kuntscher, F. Kremer,Pressure-Dependent FTIR-Spectroscopy on the Counterbalance between External and Internal Constraints in Spider Silk of Nephila pilipes, Macromolecules 46, 4919−4923 (2013)
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Anton M., Heidebrecht A., Mahmood N., Beiner M., Scheibel T., Kremer F., Foundation of the Outstanding Toughness in Biomimetic and Natural Spider Silk, Biomacromolecules 18, 3954-3962 (2017)
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