Rothemund
The goal of our service project was the synthesis and supply of labeled and unlabeled peptides that bore the tendency to aggregate during the synthesis as well as in structural analysis investigations. We aimed for purities of ≤95% in the scale of 10 mg to 200 mg and reproducible conditions for subsequent biophysical studies. Generally, the peptides were analogs from biologically active peptides such as amyloid ß 1-40 and 1-42 (Aß1-40 and Aß1-42) and parathyroid hormone 1-84 (PTH1-84). In our Core Unit custom peptide synthesis, we performed the synthesis for peptides with a length between 3 and 80 residues using state-of-the-art fully automated instruments based on Fmoc solid-phase technology, employing a microwave-assisted peptide synthesizer. We had established the know-how to select and optimize the appropriate peptide synthesis method for the supply of challenging peptide sequences. All peptides were HPLC purified, analyzed by MALDI-TOF mass spectrometry, and delivered with detailed quality control documentation, including HPLC and MS spectra. In particular, we had optimized special protocols for the incorporation of expensive isotope-labeled amino acids (replacing 12C with 13C, 14N with 15N, and/or 1H with 2H) or other labels such as stable and hydrophilic fluorescence labels (ATTO dyes) to cost-effectively prepare peptides in large quantities (up to 200 mg) with high purities (95% or higher).